Fan: Dean always wears a certain necklace. Does it mean anything?
Jensen You’re gonna hang me out to dry on this one?
Eric: Oh yeah.
Jensen: It does has significance but we can’t talk about it.
John Shiban: It’s from Sacramento.
Paley Festival March 2006.
Dean’s amulet has been an object of fascination since the Show began, and has become as important to fandom as it is – or at least was - to Dean.
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For more on the possibly mythology behind the amulet, see the Amulet page on the Super Wiki